Application of internal transcribed spacer of nuclear ribosomal DNA for identification of <i>Echinops mandavillei</i> Kit Tan
نویسندگان
چکیده
منابع مشابه
Limits of nuclear ribosomal DNA internal transcribed spacer (ITS) sequences as species barcodes for Fungi.
In PNAS, the work by Schoch et al. (1) proposed nuclear ribosomal DNA internal transcribed spacer (ITS) sequences as the sole universal barcode for fungi. The work by Schoch et al. (1) stated that “the proposal will satisfy most fungal biologists but not all” (1). I am among those biologists who are not so satisfied and not really surprised either, because ITS has been the most widely used mark...
متن کاملExtensive Pyrosequencing Reveals Frequent Intra-Genomic Variations of Internal Transcribed Spacer Regions of Nuclear Ribosomal DNA
BACKGROUND Internal transcribed spacer of nuclear ribosomal DNA (nrDNA) is already one of the most popular phylogenetic and DNA barcoding markers. However, the existence of its multiple copies has complicated such usage and a detailed characterization of intra-genomic variations is critical to address such concerns. METHODOLOGY/PRINCIPAL FINDINGS In this study, we used sequence-tagged pyroseq...
متن کاملThe internal transcribed spacer of nuclear ribosomal DNA in the gymnosperm Gnetum.
We analyze the structure of the internal transcribed spacers ITS1 and ITS2 of the nuclear ribosomal DNA in the gymnosperm Gnetum, using a phylogenetic framework derived mainly from an intron in the nuclear low-copy LEAFY gene. Gnetum comprises 25-35 species in South America, Africa, and Asia, of which we sampled 16, each with two to six clones. Criteria used to assess ITS functionality were hig...
متن کاملNuclear ribosomal internal transcribed spacer (ITS) region as a universal DNA barcode marker for Fungi.
Six DNA regions were evaluated as potential DNA barcodes for Fungi, the second largest kingdom of eukaryotic life, by a multinational, multilaboratory consortium. The region of the mitochondrial cytochrome c oxidase subunit 1 used as the animal barcode was excluded as a potential marker, because it is difficult to amplify in fungi, often includes large introns, and can be insufficiently variabl...
متن کاملRibosomal Internal Transcribed Spacer of Prototheca wickerhamii Has Characteristic Structure Useful for Identification and Genotyping
Prototheca species are achlorophyllous algae ubiquitous in nature and known to cause localized and systemic infection both in humans and animals. Although identification of the Prototheca species in clinical specimens is a challenge, there are an increasing number of cases in which molecular techniques have successfully been used for diagnosis of protothecosis. In this study, we characterized n...
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ژورنال
عنوان ژورنال: Bangladesh Journal of Plant Taxonomy
سال: 2014
ISSN: 2224-7297,1028-2092
DOI: 10.3329/bjpt.v21i1.19256